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2 ml evt basal medium  (Thermo Fisher)


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    Thermo Fisher 2 ml evt basal medium
    ( A ) The experimental scheme for <t>EVT</t> differentiation from naïve <t>hPSC-derived</t> <t>hTSCs.</t> ( B ) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 μm. ( C ) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( D ) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. ( E ) Quantitative gene expression analysis for EVT marker gene MMP2 in H9 and AN_1.1 hTSC-like cells and EVT-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001. ( F ) Matrigel invasion assay of H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( G ) The experimental scheme for STB differentiation from naïve hPSC-derived hTSCs. ( H ) Phase contrast image of H9 and AN_1.1 hTSC-like and 3D STB-like cells. The scale bars indicate 75 μm. ( I ) Quantitative gene expression analysis for STB marker genes CGB and SDC1 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ±1 SD of technical replicates. ‘*' indicates a p-value<0.05, and ‘**' indicates a p-value<0.01. ( J ) Immunofluorescence staining for STB markers hCG and SDC1 in H9 3D STB-like cells. The scale bars indicate 75 μm. ( K ) Immunofluorescence staining for STB markers hCG and SDC1 in AN_1.1 3D STB-like cells. The scale bars indicate 75 μm. ( L ) Quantitative gene expression analysis for TSC marker gene TEAD4 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001.
    2 Ml Evt Basal Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+ml+evt+basal+medium/pmc07062471-313-12-37
    Average 90 stars, based on 1 article reviews
    2 ml evt basal medium - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Derivation of trophoblast stem cells from naïve human pluripotent stem cells"

    Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells

    Journal: eLife

    doi: 10.7554/eLife.52504

    ( A ) The experimental scheme for EVT differentiation from naïve hPSC-derived hTSCs. ( B ) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 μm. ( C ) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( D ) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. ( E ) Quantitative gene expression analysis for EVT marker gene MMP2 in H9 and AN_1.1 hTSC-like cells and EVT-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001. ( F ) Matrigel invasion assay of H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( G ) The experimental scheme for STB differentiation from naïve hPSC-derived hTSCs. ( H ) Phase contrast image of H9 and AN_1.1 hTSC-like and 3D STB-like cells. The scale bars indicate 75 μm. ( I ) Quantitative gene expression analysis for STB marker genes CGB and SDC1 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ±1 SD of technical replicates. ‘*' indicates a p-value<0.05, and ‘**' indicates a p-value<0.01. ( J ) Immunofluorescence staining for STB markers hCG and SDC1 in H9 3D STB-like cells. The scale bars indicate 75 μm. ( K ) Immunofluorescence staining for STB markers hCG and SDC1 in AN_1.1 3D STB-like cells. The scale bars indicate 75 μm. ( L ) Quantitative gene expression analysis for TSC marker gene TEAD4 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001.
    Figure Legend Snippet: ( A ) The experimental scheme for EVT differentiation from naïve hPSC-derived hTSCs. ( B ) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 μm. ( C ) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( D ) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. ( E ) Quantitative gene expression analysis for EVT marker gene MMP2 in H9 and AN_1.1 hTSC-like cells and EVT-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001. ( F ) Matrigel invasion assay of H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( G ) The experimental scheme for STB differentiation from naïve hPSC-derived hTSCs. ( H ) Phase contrast image of H9 and AN_1.1 hTSC-like and 3D STB-like cells. The scale bars indicate 75 μm. ( I ) Quantitative gene expression analysis for STB marker genes CGB and SDC1 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ±1 SD of technical replicates. ‘*' indicates a p-value<0.05, and ‘**' indicates a p-value<0.01. ( J ) Immunofluorescence staining for STB markers hCG and SDC1 in H9 3D STB-like cells. The scale bars indicate 75 μm. ( K ) Immunofluorescence staining for STB markers hCG and SDC1 in AN_1.1 3D STB-like cells. The scale bars indicate 75 μm. ( L ) Quantitative gene expression analysis for TSC marker gene TEAD4 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001.

    Techniques Used: Derivative Assay, Flow Cytometry, Marker, Enzyme-linked Immunosorbent Assay, Expressing, Invasion Assay, Immunofluorescence, Staining

    Related Articles

    Derivative Assay:

    Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells
    Article Snippet: EVT differentiation, 6-well plates were coated with 1 μg/mL Collagen IV overnight. .. 0.75 × 10 5 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM β-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 μM A83-01, 2.5 μM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC). .. Matrigel was added to a 2% final concentration shortly after resuspending hTSCs in the

    Flow Cytometry:

    Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells
    Article Snippet: EVT differentiation, 6-well plates were coated with 1 μg/mL Collagen IV overnight. .. 0.75 × 10 5 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM β-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 μM A83-01, 2.5 μM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC). .. Matrigel was added to a 2% final concentration shortly after resuspending hTSCs in the

    Marker:

    Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells
    Article Snippet: EVT differentiation, 6-well plates were coated with 1 μg/mL Collagen IV overnight. .. 0.75 × 10 5 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM β-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 μM A83-01, 2.5 μM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC). .. Matrigel was added to a 2% final concentration shortly after resuspending hTSCs in the

    Enzyme-linked Immunosorbent Assay:

    Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells
    Article Snippet: EVT differentiation, 6-well plates were coated with 1 μg/mL Collagen IV overnight. .. 0.75 × 10 5 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM β-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 μM A83-01, 2.5 μM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC). .. Matrigel was added to a 2% final concentration shortly after resuspending hTSCs in the

    Expressing:

    Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells
    Article Snippet: EVT differentiation, 6-well plates were coated with 1 μg/mL Collagen IV overnight. .. 0.75 × 10 5 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM β-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 μM A83-01, 2.5 μM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC). .. Matrigel was added to a 2% final concentration shortly after resuspending hTSCs in the

    Invasion Assay:

    Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells
    Article Snippet: EVT differentiation, 6-well plates were coated with 1 μg/mL Collagen IV overnight. .. 0.75 × 10 5 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM β-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 μM A83-01, 2.5 μM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC). .. Matrigel was added to a 2% final concentration shortly after resuspending hTSCs in the

    Immunofluorescence:

    Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells
    Article Snippet: EVT differentiation, 6-well plates were coated with 1 μg/mL Collagen IV overnight. .. 0.75 × 10 5 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM β-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 μM A83-01, 2.5 μM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC). .. Matrigel was added to a 2% final concentration shortly after resuspending hTSCs in the

    Staining:

    Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells
    Article Snippet: EVT differentiation, 6-well plates were coated with 1 μg/mL Collagen IV overnight. .. 0.75 × 10 5 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM β-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 μM A83-01, 2.5 μM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC). .. Matrigel was added to a 2% final concentration shortly after resuspending hTSCs in the



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    2 ml evt basal medium  (Thermo Fisher)
    90
    Thermo Fisher 2 ml evt basal medium
    ( A ) The experimental scheme for <t>EVT</t> differentiation from naïve <t>hPSC-derived</t> <t>hTSCs.</t> ( B ) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 μm. ( C ) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( D ) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. ( E ) Quantitative gene expression analysis for EVT marker gene MMP2 in H9 and AN_1.1 hTSC-like cells and EVT-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001. ( F ) Matrigel invasion assay of H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( G ) The experimental scheme for STB differentiation from naïve hPSC-derived hTSCs. ( H ) Phase contrast image of H9 and AN_1.1 hTSC-like and 3D STB-like cells. The scale bars indicate 75 μm. ( I ) Quantitative gene expression analysis for STB marker genes CGB and SDC1 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ±1 SD of technical replicates. ‘*' indicates a p-value<0.05, and ‘**' indicates a p-value<0.01. ( J ) Immunofluorescence staining for STB markers hCG and SDC1 in H9 3D STB-like cells. The scale bars indicate 75 μm. ( K ) Immunofluorescence staining for STB markers hCG and SDC1 in AN_1.1 3D STB-like cells. The scale bars indicate 75 μm. ( L ) Quantitative gene expression analysis for TSC marker gene TEAD4 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001.
    2 Ml Evt Basal Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+ml+evt+basal+medium/pmc07062471-313-12-37
    Average 90 stars, based on 1 article reviews
    2 ml evt basal medium - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) The experimental scheme for EVT differentiation from naïve hPSC-derived hTSCs. ( B ) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 μm. ( C ) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( D ) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. ( E ) Quantitative gene expression analysis for EVT marker gene MMP2 in H9 and AN_1.1 hTSC-like cells and EVT-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001. ( F ) Matrigel invasion assay of H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( G ) The experimental scheme for STB differentiation from naïve hPSC-derived hTSCs. ( H ) Phase contrast image of H9 and AN_1.1 hTSC-like and 3D STB-like cells. The scale bars indicate 75 μm. ( I ) Quantitative gene expression analysis for STB marker genes CGB and SDC1 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ±1 SD of technical replicates. ‘*' indicates a p-value<0.05, and ‘**' indicates a p-value<0.01. ( J ) Immunofluorescence staining for STB markers hCG and SDC1 in H9 3D STB-like cells. The scale bars indicate 75 μm. ( K ) Immunofluorescence staining for STB markers hCG and SDC1 in AN_1.1 3D STB-like cells. The scale bars indicate 75 μm. ( L ) Quantitative gene expression analysis for TSC marker gene TEAD4 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001.

    Journal: eLife

    Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells

    doi: 10.7554/eLife.52504

    Figure Lengend Snippet: ( A ) The experimental scheme for EVT differentiation from naïve hPSC-derived hTSCs. ( B ) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 μm. ( C ) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( D ) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. ( E ) Quantitative gene expression analysis for EVT marker gene MMP2 in H9 and AN_1.1 hTSC-like cells and EVT-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001. ( F ) Matrigel invasion assay of H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( G ) The experimental scheme for STB differentiation from naïve hPSC-derived hTSCs. ( H ) Phase contrast image of H9 and AN_1.1 hTSC-like and 3D STB-like cells. The scale bars indicate 75 μm. ( I ) Quantitative gene expression analysis for STB marker genes CGB and SDC1 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ±1 SD of technical replicates. ‘*' indicates a p-value<0.05, and ‘**' indicates a p-value<0.01. ( J ) Immunofluorescence staining for STB markers hCG and SDC1 in H9 3D STB-like cells. The scale bars indicate 75 μm. ( K ) Immunofluorescence staining for STB markers hCG and SDC1 in AN_1.1 3D STB-like cells. The scale bars indicate 75 μm. ( L ) Quantitative gene expression analysis for TSC marker gene TEAD4 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001.

    Article Snippet: 0.75 × 10 5 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM β-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 μM A83-01, 2.5 μM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC).

    Techniques: Derivative Assay, Flow Cytometry, Marker, Enzyme-linked Immunosorbent Assay, Expressing, Invasion Assay, Immunofluorescence, Staining